| Gene name |
SPBC20F10.10 |
| Gene ID |
08/G10 |
| Gene synonyms/obsolete |
|
| Gene product |
Pho85p-associated
Cyclin; involved in regulation of glycogen metabolism |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
732 |
| ORF length (spliced) |
|
| Entry clone length |
732 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC20F10.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTTGGCGTTTACGCT |
| Rev primer name |
SPBC20F10.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACCAATTGAGTGAGAAGAG |
| Amino acid length |
243 |
| Molecular weight |
27.9 |
| Isoelectric point (calc.) |
5.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHELNHLEL |
| Localization (YFP) |
SPB;
nucleus>cytosol; nuclear dots; periphery at site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |