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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC25H1.04
Gene ID 08/G12
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 735
ORF length (spliced)
Entry clone length 735
No. of intron 0
Sequence status Finished
Sequence results (-8)G:addition
Comments 5' terminus is frameshifted.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC25H1.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAAAATGCTTGCAGCA
Rev primer name SPAC25H1.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGTCCGAAATTCTGGTA
Amino acid length 244
Molecular weight 28
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Leica

Image information
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