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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1687.17c
Gene ID 08/H12
Gene synonyms/obsolete
Gene product Der1-like (degradation in the ER) family; no apparent Sc ortholog; non-essential
Entry clone Cloned#
ORF length (unspliced) 741
ORF length (spliced) 507
Entry clone length 741
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1687.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAATTTTGCCAGAATT
Rev primer name SPAC1687.17.Rv
Rev primer SEQ AGAAAGCTGGGTATACTGTAGAAAAATCTGTA
Amino acid length 168
Molecular weight 19.6
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain 5
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYRISILGL/LRTGIFPLLDL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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