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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.06
Gene ID 09/C08
Gene synonyms/obsolete
Gene product fungal conserved protein; similar to Sp SPCC757.03C
Entry clone Cloned
ORF length (unspliced) 756
ORF length (spliced)
Entry clone length 756
No. of intron 0
Sequence status Finished
Sequence results 655T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F7.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGAACGACATGAAGC
Rev primer name SPAC1F7.06.Rv
Rev primer SEQ AGAAAGCTGGGTATCCATCATAATTTAACACA
Amino acid length 251
Molecular weight 27.9
Isoelectric point (calc.) 4.2
Signal SEQ
No. of transmembrane domain 9
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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