Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC9E9.13
Gene ID 09/D02
Gene synonyms/obsolete wos2
Gene product p23 homolog; Hsp associated co-chaperone; interacts physically with Hsp90p; involved in mitotic control; interacts with Wee1p; interacts physically with Cdc2p; 3' UTR directs the formation multiple mRNAs; alternative polyadenylation sites results in the production of the 3 differently sized transcripts; alternative transcripts
Entry clone Cloned#
ORF length (unspliced) 760
ORF length (spliced) 561
Entry clone length 760
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC9E9.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTTTAAATACACAAAT
Rev primer name SPAC9E9.13.Rv
Rev primer SEQ AGAAAGCTGGGTACTCTTTCTTTTCGTTACTG
Amino acid length 186
Molecular weight 20.9
Isoelectric point (calc.) 4.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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