| Gene name |
SPBC839.04 |
| Gene ID |
09/D05 |
| Gene synonyms/obsolete |
rpl803; rpk5-b; rpkD4;
rpl8-3 |
| Gene product |
60S ribosomal protein
L8 |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
762 |
| ORF length (spliced) |
|
| Entry clone length |
762 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
6T:A |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC839.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGACGTGTTATTAGAGC |
| Rev primer name |
SPBC839.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTTTCGACAGCAGCAGCA |
| Amino acid length |
253 |
| Molecular weight |
27.1 |
| Isoelectric point (calc.) |
11.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol |
| Microscope used for
observation |
DeltaVision |