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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC2G5.02c
Gene ID 09/D12
Gene synonyms/obsolete
Gene product casein kinase II (beta subunit); similar to Sp ckb1
Entry clone Cloned
ORF length (unspliced) 765
ORF length (spliced)
Entry clone length 765
No. of intron 0
Sequence status Finished
Sequence results 38C:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC2G5.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGCGAAAAAACCTCAT
Rev primer name SPBC2G5.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATCACTTTCAGACTCCGAA
Amino acid length 254
Molecular weight 29
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFNLTGLNL
Localization (YFP) nucleus>cytosol; SPB?
Comments for localization nuclear dots by over expression; 1~2 dots/nucleus
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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