Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC359.06
Gene ID 09/E12
Gene synonyms/obsolete
Gene product class II aldolase and adducin N terminal domain; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 774
ORF length (spliced)
Entry clone length 774
No. of intron 0
Sequence status Finished
Sequence results 330T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC359.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAAAGTAACTGGATT
Rev primer name SPBC359.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAATTATTTCTAGTTCCCCA
Amino acid length 257
Molecular weight 28.8
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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