| Gene name |
SPBC1861.08c |
| Gene ID |
09/F03 |
| Gene synonyms/obsolete |
|
| Gene product |
U2 snRNP complex;
complexed with Cdc5p; putative U2 snRNP-specific A' protein;
involved in mRNA splicing (initial steps); leucine-rich repeat
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
775 |
| ORF length (spliced) |
720 |
| Entry clone length |
775 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1861.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTCTTAATGCTGAATT |
| Rev primer name |
SPBC1861.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTGGAATTTTCCCCTCT |
| Amino acid length |
239 |
| Molecular weight |
27.2 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPNLKTLSL/LIWRIPSLHI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |