| Gene name |
SPBC409.04c |
| Gene ID |
09/G06 |
| Gene synonyms/obsolete |
mis12 |
| Gene product |
inner centromere
protein; involved in chromosome segregation (required);
involved in centromere structure (maintenance)
(required) |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
780 |
| ORF length (spliced) |
|
| Entry clone length |
780 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC409.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTAGTGGAGTTACTAGA |
| Rev primer name |
SPBC409.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGCTCGTCAGTATGTCCG |
| Amino acid length |
259 |
| Molecular weight |
30.1 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
126 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; moving
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |