Gene name |
SPAC607.02c |
Gene ID |
10/A02 |
Gene synonyms/obsolete |
|
Gene product |
lysine-rich protein;
hypothetical protein; sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
790 |
ORF length (spliced) |
696 |
Entry clone length |
790 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
408T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC607.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTACTAAGACTAAGAA |
Rev primer name |
SPAC607.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTCCGCTTGGTTTTCATT |
Amino acid length |
231 |
Molecular weight |
27.3 |
Isoelectric point (calc.) |
10.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
50/115/3 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|