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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC32F12.05c
Gene ID 10/C04
Gene synonyms/obsolete cwf12; isy1
Gene product involved in mRNA splicing; complexed with Cdc5p
Entry clone Cloned
ORF length (unspliced) 806
ORF length (spliced) 654
Entry clone length 806
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC32F12.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCATCAGCGAAAGTAAG
Rev primer name SPBC32F12.05.Rv
Rev primer SEQ AGAAAGCTGGGTATGAAATTCTGCTCAGCAAA
Amino acid length 217
Molecular weight 25.6
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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