| Gene name |
SPAC1B3.14 |
| Gene ID |
10/C12 |
| Gene synonyms/obsolete |
vma3 |
| Gene product |
V-type ATPase;
vacuolar ATP synthase subunit; V0 sector; proteolipid
subunit |
| Entry clone |
Cloned |
| ORF length (unspliced) |
810 |
| ORF length (spliced) |
486 |
| Entry clone length |
810 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1B3.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTACAGATCTTTGGTA |
| Rev primer name |
SPAC1B3.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCACTAAAGAGGGTTAGCC |
| Amino acid length |
161 |
| Molecular weight |
16.3 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
4 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LILIFAEVLGL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|