| Gene name |
SPBC1105.09 |
| Gene ID |
10/D02 |
| Gene synonyms/obsolete |
ubc15 |
| Gene product |
ubiquitin conjugating
enzyme; involved in the regulation of transcriptional
silencing of heterochromatin; no apparent Sc ortholog;
involved in transcriptional silencing of the mating-type
region; overexpression derepressed transcriptional silencing
of the mating-type region |
| Entry clone |
Cloned |
| ORF length (unspliced) |
810 |
| ORF length (spliced) |
504 |
| Entry clone length |
810 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
328G:A / 700A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1105.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTTCTTCTGCTAGTGA |
| Rev primer name |
SPBC1105.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATCATTTCAATTGACCTG |
| Amino acid length |
167 |
| Molecular weight |
19.1 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|