Gene name |
SPAC17C9.10 |
Gene ID |
10/E03 |
Gene synonyms/obsolete |
stm1 |
Gene product |
G-protein coupled
receptor activity; possibly couples with heterotrimeric G
protein Gpa2p; PQ loop; similar to Sp SPAC2E12.03c |
Entry clone |
Cloned |
ORF length (unspliced) |
816 |
ORF length (spliced) |
|
Entry clone length |
816 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17C9.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTGATAGCTCCTTC |
Rev primer name |
SPAC17C9.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATAATGATTACGATACTTG |
Amino acid length |
271 |
Molecular weight |
30.6 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
7 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSFLGALSL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal
|