Gene name |
SPBC17D11.03c |
Gene ID |
10/E07 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
818 |
ORF length (spliced) |
642 |
Entry clone length |
818 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
162A:G / 378A:G /
678A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC17D11.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCCATAACACTATGCT |
Rev primer name |
SPBC17D11.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAGGGTTTCTATATCTCCT |
Amino acid length |
213 |
Molecular weight |
23.4 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|