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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17C9.02c
Gene ID 10/E11
Gene synonyms/obsolete lys7
Gene product alpha-aminoadipate reductase-phosphopantetheinyl transferase; involved in lysine biosynthesis (6th step); ACPS domain
Entry clone Cloned (also cloned in 2004 trial)
ORF length (unspliced) 820
ORF length (spliced) 777
Entry clone length 820
No. of intron 1
Sequence status Finished
Sequence results 420A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17C9.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAACAAAAAGTATATAG
Rev primer name SPAC17C9.02.Rv
Rev primer SEQ AGAAAGCTGGGTACAAGTCGTTCAATGTCTCC
Amino acid length 258
Molecular weight 29.3
Isoelectric point (calc.) 5.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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