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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC13G1.04c
Gene ID 10/F05
Gene synonyms/obsolete
Gene product alkB homolog; involved in DNA repair; 2 OG-Fe(II) oxygenase superfamily; no apparent Sc ortholog; non-essential
Entry clone Cloned
ORF length (unspliced) 822
ORF length (spliced)
Entry clone length 822
No. of intron 0
Sequence status Finished
Sequence results 401A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC13G1.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACAGGCGAATGTTTT
Rev primer name SPBC13G1.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGTAAGCAATCAATTGACTA
Amino acid length 273
Molecular weight 31.1
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSPFYQLPL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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