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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.13
Gene ID 10/G03
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 831
ORF length (spliced) 756
Entry clone length 831
No. of intron 1
Sequence status Finished
Sequence results 397T:C / 774T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP8B7.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGAGCCCAAGCCTAT
Rev primer name SPBP8B7.13.Rv
Rev primer SEQ AGAAAGCTGGGTATAACAACGGTTGGACTGCA
Amino acid length 251
Molecular weight 27.7
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRNLLHKRLWI
Localization (YFP) ER; Golgi?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal, DeltaVision

Image information
YFP 4 images) See all images

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