| Gene name |
SPAC5D6.05 |
| Gene ID |
10/G05 |
| Gene synonyms/obsolete |
sep11; pmc6 |
| Gene product |
RNA polymerase II
holoenzyme component; mediator complex subunit; involved in
cytokinesis; involved in cell separation; involved in sexual
differentiation (required); no apparent orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
832 |
| ORF length (spliced) |
624 |
| Entry clone length |
832 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
138A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC5D6.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAGAACTGTATCTTTT |
| Rev primer name |
SPAC5D6.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAATCTGACGTTTTTTAAA |
| Amino acid length |
207 |
| Molecular weight |
24 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|