Gene name |
SPCC830.06 |
Gene ID |
10/G10 |
Gene synonyms/obsolete |
|
Gene product |
calcineurin b subunit;
protein phosphatase regulatory subunit; EF hand motifs;
calcium binding protein |
Entry clone |
Cloned |
ORF length (unspliced) |
833 |
ORF length (spliced) |
525 |
Entry clone length |
833 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
22A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC830.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTCAGTCGCAGTCGCA |
Rev primer name |
SPCC830.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAAGAATCGAGTGTCATG |
Amino acid length |
174 |
Molecular weight |
19.6 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|