Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1B2.04
Gene ID 10/G12
Gene synonyms/obsolete cox6
Gene product cytochrome c oxidase (subunit VI)
Entry clone Cloned
ORF length (unspliced) 834
ORF length (spliced) 423
Entry clone length 834
No. of intron 1
Sequence status Finished
Sequence results 434A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1B2.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAGCAGTTCAACGTAT
Rev primer name SPAC1B2.04.Rv
Rev primer SEQ AGAAAGCTGGGTACTTAAAAAGATCCTCCTTC
Amino acid length 140
Molecular weight 15.9
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKPVCNELGI
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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