Gene name |
SPBC800.02 |
Gene ID |
10/H07 |
Gene synonyms/obsolete |
|
Gene product |
serine-rich protein;
hypothetical protein; sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
837 |
ORF length (spliced) |
759 |
Entry clone length |
837 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC800.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGGCGGCCACAAGAA |
Rev primer name |
SPBC800.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGGTTTTTTCGAAGGCATA |
Amino acid length |
252 |
Molecular weight |
28.4 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
one nuclear dot by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |