Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC800.02
Gene ID 10/H07
Gene synonyms/obsolete
Gene product serine-rich protein; hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 837
ORF length (spliced) 759
Entry clone length 837
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC800.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAGGCGGCCACAAGAA
Rev primer name SPBC800.02.Rv
Rev primer SEQ AGAAAGCTGGGTATGGTTTTTTCGAAGGCATA
Amino acid length 252
Molecular weight 28.4
Isoelectric point (calc.) 10.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization one nuclear dot by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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