| Gene name |
SPBC1709.04c |
| Gene ID |
11/A01 |
| Gene synonyms/obsolete |
cyp3 |
| Gene product |
cyclophilin;
U-snRNP-associated; involved in mRNA splicing; peptidyl-prolyl
cis-trans isomerase; functional ortholog of human USA-Cyp; no
apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
838 |
| ORF length (spliced) |
522 |
| Entry clone length |
838 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1709.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCACCGAACCTGTTGT |
| Rev primer name |
SPBC1709.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATTTCTCCACATTCGACT |
| Amino acid length |
173 |
| Molecular weight |
18.9 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |