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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC688.02c
Gene ID 11/A09
Gene synonyms/obsolete
Gene product spindle pole body component; involved in chromosome segregation
Entry clone Cloned
ORF length (unspliced) 848
ORF length (spliced) 633
Entry clone length 848
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC688.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTCTCACGGAGATGC
Rev primer name SPAC688.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGAGTTTTTTCCAATCGG
Amino acid length 210
Molecular weight 24.1
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus; SPB
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (SPB?)
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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