Gene name |
SPAC1071.09c |
Gene ID |
11/A12 |
Gene synonyms/obsolete |
|
Gene product |
DNAJ domain
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
849 |
ORF length (spliced) |
|
Entry clone length |
849 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
77T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1071.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGGTTTACTCAATCT |
Rev primer name |
SPAC1071.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCCTTGGATCGTGATTTT |
Amino acid length |
282 |
Molecular weight |
32.8 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
198/228/199 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |