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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19G12.09
Gene ID 11/C05
Gene synonyms/obsolete
Gene product oxidoreductase; aldo/keto reductase
Entry clone Cloned
ORF length (unspliced) 855
ORF length (spliced)
Entry clone length 855
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19G12.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTATCGCTGCTATGGG
Rev primer name SPAC19G12.09.Rv
Rev primer SEQ AGAAAGCTGGGTACTCATCCATGTGCTTCATA
Amino acid length 284
Molecular weight 31.5
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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