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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC337.06c
Gene ID 11/D01
Gene synonyms/obsolete cwf15
Gene product complexed with Cdc5p; involved in mRNA splicing
Entry clone Cloned
ORF length (unspliced) 857
ORF length (spliced) 798
Entry clone length 857
No. of intron 1
Sequence status Finished
Sequence results 89T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC337.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACGACTGCACATGTATG
Rev primer name SPBC337.06.Rv
Rev primer SEQ AGAAAGCTGGGTAATCAACAAATCTTGCCAAG
Amino acid length 265
Molecular weight 30.4
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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