| Gene name |
SPCC320.11c |
| Gene ID |
11/D07 |
| Gene synonyms/obsolete |
SPCC330.18 |
| Gene product |
involved in 60S
ribosomal subunit biogenesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
858 |
| ORF length (spliced) |
543 |
| Entry clone length |
858 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
219A:G / 689C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC320.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGGCCTTTAACTCATGA |
| Rev primer name |
SPCC320.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAAAGAGTATCTTCATCA |
| Amino acid length |
180 |
| Molecular weight |
20.8 |
| Isoelectric point (calc.) |
9.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |