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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3A12.12
Gene ID 11/E03
Gene synonyms/obsolete atp11
Gene product F1 ATPase chaperone; ATP synthesis coupled proton transport; involved in mitochondrial F1-F0 ATPase assembly
Entry clone Cloned
ORF length (unspliced) 861
ORF length (spliced)
Entry clone length 861
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3A12.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTCCCATATGGAAACT
Rev primer name SPAC3A12.12.Rv
Rev primer SEQ AGAAAGCTGGGTATTCCAACATATCCATATGC
Amino acid length 286
Molecular weight 32.8
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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