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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1296.05c
Gene ID 11/E12
Gene synonyms/obsolete
Gene product cyclin related protein
Entry clone Cloned
ORF length (unspliced) 865
ORF length (spliced) 777
Entry clone length 865
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1296.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAGATTCTTTGGTACA
Rev primer name SPAC1296.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAGAAACTTGCTCAGTA
Amino acid length 258
Molecular weight 29.3
Isoelectric point (calc.) 5.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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