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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19A8.11c
Gene ID 11/F03
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 866
ORF length (spliced) 741
Entry clone length 866
No. of intron 3
Sequence status Finished
Sequence results 97A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19A8.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGAGTTATTTTGGTTAT
Rev primer name SPAC19A8.11.Rv
Rev primer SEQ AGAAAGCTGGGTACAGCGATTCATAATCATTT
Amino acid length 246
Molecular weight 28.2
Isoelectric point (calc.) 4.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFKNFIDKLQL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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