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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3C7.10
Gene ID 11/F07
Gene synonyms/obsolete
Gene product src (SH3) homology domain; interacts physically with Pex5p and Pex14p
Entry clone Cloned
ORF length (unspliced) 867
ORF length (spliced)
Entry clone length 867
No. of intron 0
Sequence status Finished
Sequence results 667A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3C7.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAACAAATCAAAATGA
Rev primer name SPAC3C7.10.Rv
Rev primer SEQ AGAAAGCTGGGTACCTTGAAATGATTGAACAA
Amino acid length 288
Molecular weight 32
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLSRLKRLVL
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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