Gene name |
SPAC6G9.07c |
Gene ID |
11/G09 |
Gene synonyms/obsolete |
arp10 |
Gene product |
ARP2/3
actin-organizing complex; involved in actin assembly and
function; involved in actin cortical patch assembly |
Entry clone |
Cloned |
ORF length (unspliced) |
873 |
ORF length (spliced) |
507 |
Entry clone length |
873 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
91T:C / 355A:G /
781A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6G9.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGAGAGGAATTCTT |
Rev primer name |
SPAC6G9.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAACAACTCAGATAAGTT |
Amino acid length |
168 |
Molecular weight |
19.6 |
Isoelectric point (calc.) |
5.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |