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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22E12.13c
Gene ID 11/H05
Gene synonyms/obsolete rpl2403; rpl24-3
Gene product 60S ribosomal protein L24-3 (L30)
Entry clone Cloned
ORF length (unspliced) 876
ORF length (spliced) 579
Entry clone length 876
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22E12.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGGTTTGTAGAGCTAAA
Rev primer name SPAC22E12.13.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCCATTTGCATGGCTGAA
Amino acid length 192
Molecular weight 22.2
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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