Gene name |
SPBC1711.09c |
Gene ID |
11/H07 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
879 |
ORF length (spliced) |
813 |
Entry clone length |
879 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
76T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1711.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTGCTCCAGTATCTTC |
Rev primer name |
SPBC1711.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTGTTGATGACTTAACAAA |
Amino acid length |
270 |
Molecular weight |
30 |
Isoelectric point (calc.) |
6.1 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
5 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPMSFLIALVL |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |