Gene name |
SPBC25H2.06c |
Gene ID |
11/H11 |
Gene synonyms/obsolete |
hrf1 |
Gene product |
involved in
intracellular protein transport; involved in ER to Golgi
transport; interacts physically with SPCC61.04c; complexed
with SPCC61.04c; COPII coated vesicle component |
Entry clone |
Cloned |
ORF length (unspliced) |
882 |
ORF length (spliced) |
|
Entry clone length |
882 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC25H2.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCACCCAAGTTGTACCA |
Rev primer name |
SPBC25H2.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGACAGCACATACATGAAT |
Amino acid length |
293 |
Molecular weight |
33 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
|
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |