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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19G12.12
Gene ID 12/A02
Gene synonyms/obsolete coq1
Gene product hexaprenyl pyrophosphate synthetase; involved in ubiquinone biosynthesis
Entry clone Cloned
ORF length (unspliced) 885
ORF length (spliced)
Entry clone length 885
No. of intron 0
Sequence status Finished
Sequence results 232A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19G12.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCTTTCCGTTCGCTAG
Rev primer name SPAC19G12.12.Rv
Rev primer SEQ AGAAAGCTGGGTACTTCGAAACACTATTAGCG
Amino acid length 294
Molecular weight 32.4
Isoelectric point (calc.) 8.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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