| Gene name |
SPBP4H10.16c |
| Gene ID |
12/A10 |
| Gene synonyms/obsolete |
|
| Gene product |
RAD3 epistasis group;
involved in DNA repair; involved in mismatch repair; similar
to Sp SPAC20H4.08 |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
888 |
| ORF length (spliced) |
|
| Entry clone length |
888 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBP4H10.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTGATAACTCAGGC |
| Rev primer name |
SPBP4H10.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACCGCTGCAGTCTCGTAC |
| Amino acid length |
295 |
| Molecular weight |
33.7 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMCLFPRGLLL |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
| Microscope used for
observation |
DeltaVision |