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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC30D11.13
Gene ID 12/B08
Gene synonyms/obsolete hus5; ubc9
Gene product experimentally characterised; ubiquitin conjugating enzyme; involved in mitosis (required); interacts physically with Rad51p; SUMO conjugator; involved in S phase arrest recovery (required)
Entry clone Cloned
ORF length (unspliced) 894
ORF length (spliced) 474
Entry clone length 894
No. of intron 5
Sequence status Finished
Sequence results 242T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC30D11.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCATCTCTTTGTAAAAC
Rev primer name SPAC30D11.13.Rv
Rev primer SEQ AGAAAGCTGGGTATGGAGCATTTTCACGAGCC
Amino acid length 157
Molecular weight 17.9
Isoelectric point (calc.) 9.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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