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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A10.03
Gene ID 12/C08
Gene synonyms/obsolete cyp1
Gene product experimentally characterised; cyclophilin; peptidyl-prolyl cis-trans isomerase; interacts with SNW/SKIP coregulator; interacts physically with Snw1p; overexpression suppresses
Entry clone Cloned
ORF length (unspliced) 899
ORF length (spliced) 468
Entry clone length 899
No. of intron 5
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC57A10.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAATGTAGAGTTACA
Rev primer name SPAC57A10.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGTGCAACAGCTTTAATG
Amino acid length 155
Molecular weight 16.8
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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