| Gene name |
SPBC409.10 |
| Gene ID |
12/D01 |
| Gene synonyms/obsolete |
ade7 |
| Gene product |
phosphoribosylamidoimidazole-succinocarboxamide
synthase (putative); SAICAR synthetase; involved in nucleotide
metabolism; involved in purine biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
900 |
| ORF length (spliced) |
|
| Entry clone length |
900 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
294T:C / 549A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC409.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATTATTAAAAACTTC |
| Rev primer name |
SPBC409.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTAAGACCACTTCTTTCCA |
| Amino acid length |
299 |
| Molecular weight |
33.9 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |