Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC630.08c
Gene ID 12/D07
Gene synonyms/obsolete
Gene product C-4 methyl sterol oxidase; involved in ergosterol biosynthesis; sterol desaturase domain
Entry clone Cloned
ORF length (unspliced) 903
ORF length (spliced)
Entry clone length 903
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC630.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATACTACTTCTGAAGT
Rev primer name SPAC630.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTTAGCCTTTTTGCTTTCA
Amino acid length 300
Molecular weight 35.8
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNFVEQLWL
Localization (YFP) ER
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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