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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC970.06
Gene ID 12/E12
Gene synonyms/obsolete
Gene product cargo receptor for soluble proteins; surfeit locus SURF40 family; 7 predicted transmembrane helices
Entry clone Cloned
ORF length (unspliced) 909
ORF length (spliced)
Entry clone length 909
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC970.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTCACGTTCGCCGTT
Rev primer name SPCC970.06.Rv
Rev primer SEQ AGAAAGCTGGGTAATAAATCTTTTTCTTTTCA
Amino acid length 302
Molecular weight 34.3
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLLFVCVVLML/LLIFMFLGL/LSIVGGLLYL
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal, DeltaVision

Image information
YFP 2 images) See all images

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