Gene name |
SPAC11E3.03 |
Gene ID |
12/F02 |
Gene synonyms/obsolete |
|
Gene product |
monopolin complex
component; localization kinetochore; involved in chromosome
segregation (required); predicted coiled-coil region |
Entry clone |
Cloned |
ORF length (unspliced) |
911 |
ORF length (spliced) |
768 |
Entry clone length |
911 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC11E3.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAACATCTAAAGATAG |
Rev primer name |
SPAC11E3.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTTGCAAAGCTTTCATT |
Amino acid length |
255 |
Molecular weight |
29.7 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |