Gene name |
SPCC16C4.11 |
Gene ID |
12/G05 |
Gene synonyms/obsolete |
pef1 |
Gene product |
Pho85/PhoA-like
cyclin-dependent kinase; serine/threonine protein kinase |
Entry clone |
Cloned |
ORF length (unspliced) |
918 |
ORF length (spliced) |
867 |
Entry clone length |
918 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC16C4.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACTACCAAAGGCTTGA |
Rev primer name |
SPCC16C4.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCGGTTAAAAACCAAGCA |
Amino acid length |
288 |
Molecular weight |
32.7 |
Isoelectric point (calc.) |
8.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFPTFDPLGL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |