Gene name |
SPAC13F5.04c |
Gene ID |
12/H02 |
Gene synonyms/obsolete |
|
Gene product |
very hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
924 |
ORF length (spliced) |
834 |
Entry clone length |
924 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
25T:C / 222A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC13F5.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGTTTTTTTTTTCGACT |
Rev primer name |
SPAC13F5.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCTGCAGTATGATTTCCC |
Amino acid length |
277 |
Molecular weight |
29.6 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVSSFAILRI |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal |