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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC320.06
Gene ID 12/H12
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 927
ORF length (spliced) 870
Entry clone length 927
No. of intron 1
Sequence status Finished
Sequence results 179A:G / 511A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC320.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTCTCATTTCAAATAC
Rev primer name SPCC320.06.Rv
Rev primer SEQ AGAAAGCTGGGTAACGATTTATGTTTGTTAGT
Amino acid length 289
Molecular weight 33.3
Isoelectric point (calc.) 9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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