Gene name |
SPAC3A12.03c |
Gene ID |
13/A03 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; zinc finger protein; zf-C3HC4 type (RING
finger); ubiquitin ligase (E3) |
Entry clone |
Cloned |
ORF length (unspliced) |
930 |
ORF length (spliced) |
|
Entry clone length |
930 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3A12.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAATTCCAAAAAATAG |
Rev primer name |
SPAC3A12.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAAAGTACTACTCACAATG |
Amino acid length |
309 |
Molecular weight |
35 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
96/118 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent signal
|
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Zeiss |