Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3A12.03c
Gene ID 13/A03
Gene synonyms/obsolete
Gene product conserved hypothetical; zinc finger protein; zf-C3HC4 type (RING finger); ubiquitin ligase (E3)
Entry clone Cloned
ORF length (unspliced) 930
ORF length (spliced)
Entry clone length 930
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3A12.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCAATTCCAAAAAATAG
Rev primer name SPAC3A12.03.Rv
Rev primer SEQ AGAAAGCTGGGTACAAAGTACTACTCACAATG
Amino acid length 309
Molecular weight 35
Isoelectric point (calc.) 4.8
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) 96/118
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Zeiss

Image information
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