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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC895.02
Gene ID 13/A10
Gene synonyms/obsolete mad3; SPCC1795.01c
Gene product spindle assembly checkpoint component; required for Mad2p to inhibit the anaphase-promoting complex
Entry clone Cloned
ORF length (unspliced) 933
ORF length (spliced)
Entry clone length 933
No. of intron 0
Sequence status Finished
Sequence results check
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC895.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACCATTAGATGCTGG
Rev primer name SPCC895.02.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTTTCGATACTTCCTCA
Amino acid length 310
Molecular weight 36
Isoelectric point (calc.) 7.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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